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Abstract
The PICO-TAG analysis of proteins revealed that 17 protein-bound and 18 free amino acids were present throughout the developmental stages of sunflower leaves. The total protein-bound amino acid content was much higher than total free amino acid content throughout the development of sunflower leaves. The contents of protein-bound and free amino acids as well as essential and non-essential ones displayed different patterns with leaf maturation, suggesting that total protein levels are poor predictors of the nutritive status of leaves.
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