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Abstract

Papaya (Carica papaya L., 2n = 18), a polygamous angiosperm, is a major fruit crop in tropical and subtropical regions. It is trioecious with three sex forms: male, female, and hermaphrodite, where sex determination is controlled by the XY chromosome pair with two slightly different Y chromosomes i.e. Y for male and Yh for hermaphrodite. Sex type determination in papaya, which cannot be determined either by embryo shape or morphology at the juvenile developmental stage, is an essential pre-requisite for crop improvement processes as it helps in identification of fruitful plants. Hence, molecular profiling could be used as an alternative that provides a quick and reliable identifi cation of sex types in plantlets at initial stages only. In the present study we have validated the sex linked sequence characterized amplified region (SCAR) marker W11 using PCR detection assay among different cultivars of papaya i.e. dioecious with either female or male and gynodioecious with either female or hermaphrodites and also performed a double-blind test for validating the seedlings of 84 F1 plants, which resulted in their sex determination. The assay clearly gives 800 bp band in male plants in dioecious types and hermaphrodite in gynodioecious plants.

Keywords

Carica papaya dioecious double-blind test gynodieocious papaya SCAR marker sex determination

Article Details

Author Biographies

Kanupriya Chaturvedi, Indian Institute of Horticultural Research Bangalore India 560089

Scientist

Division of Biotechnology

Padmakar Bommisetty, Indian Institute of Horticultural Research Bangalore India 560089

Senior Research Fellow

Division of Biotechnology

Arpita Pattanaik, Indian Institute of Horticultural Research Bangalore India 560089

Division of Biotechnology

Vasugi Chinnaiyan, Indian Institute of Horticultural Research Bangalore India 560089

Senior Scientist

Division of Fruit Crops

Dinesh Makki Ramachandra, Indian Institute of Horticultural Research Bangalore India 560089

Principal Scientist

Division of Fruit Crops

Aswath Chennareddy, Indian Institute of Horticultural Research Bangalore India 560089

Principal Scientist & Head

Division of Biotechnology

How to Cite
Chaturvedi, K., Bommisetty, P., Pattanaik, A., Chinnaiyan, V., Ramachandra, D. M., & Chennareddy, A. (2014). PCR detection assay for sex determination in papaya using SCAR marker. Acta Botanica Croatica, 73(2). Retrieved from https://ojs3.abc.botanic.hr/index.php/abc/article/view/840

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